INFRASTRUCTURE AND SERVICES

Scientific equipment available for the program

The groups involved in the program have a number of instrumental analytical techniques, which make up much of the necessary infrastructure to carry out the program and which are available to the rest of the groups involved. They are described below:

UAH-QA

4 capillary electrophoresis equipments with diode array (DAD) detection
Liquid chromatograph with fluorescence detection
Liquid chromatograph with DAD detection
Micro liquid chromatograph with DAD detection
Semipreparative liquid chromatograph with DAD detection
Electrospray quadrupole-time-of-flight (QTOF) Mass spectrometer
Electrospray ion trap (IT) mass spectrometer
Isoelectrofocussing system (offgel)
Gel electrophoresis system

Capillary electrophoresis equipment coupled to IT mass spectrometer

Liquid chromatograph coupled to QTOF mass spectrometer

The group has also access to different centers of support for research at the University of Alcalá, highlighting the Centre for Chemical and Microbiological Analysis (CQAB), and the Biology-Medicine Center (Crop Unit). In addition, the CQAB participates in the consortium as laboratory from REDLAB.

CSIC-AI

2 Gas chromatographs with flame ionization detector (FID)
3 Gas chromatographs coupled to quadrupole mass spectrometer

Two-dimensional gas chromatograph GC x GC, with electron capture detector
Gas chromatograph coupled to triple quadrupole mass spectrometer
Gas chromatograph with electron capture detector
Gas chromatograph coupled to mass spectrometer with chemical ionization system
Multidimensional gas chromatograph (heart-cut) with electron capture detectors
Pressurized liquid extractor
Equipment for microwave assisted extraction
UV-Vis equipment with fixed wavelength
Two-dimensional gas chromatograph GC x GC coupled to time-of-flight (TOF) mass spectrometer
HPLC system coupled to quadrupole mass spectrometer
UPLC system coupled to triple quadrupole mass spectrometer

CSIC-AA

Liquid chromatography coupled to different detectors: UV-Vis, fluorescence, refractive index, mass spectrometry (MS, MS/MS, QTOF).
Gas chromatography with flame ionization detector (FID), mass spectrometry
Capillary electrophoresis coupled to UV-Vis detection
Plate Readers (ultraviolet, visible, fluorescence)
Semi-preparative chromatography
Pilot plants and laboratory of cell cultures from ICTAN

UCM-QA

Equipments for the separation, identification and determination of trace elements and their species, organic compounds and metal nanoparticles. Equipments for proteomics study:

ICP-MS HP 7700
ICP-MS Thermo SeriesX2
AF4 2000 AT series. Postnova Analytics
ESI/MS QTOF API US MICROMASS WATERS
AAS-Zeeman
EI-LTQ-MS Thermo
Laser ablation-ICP-MS Agilent Cetac
GC-microECD-MS
LC-FL Thermo P4000
CV-AFS

Equipments for sample preparation:

Laminar flow cabinet
Electrophoresis OFFGEL
Concentrator
Centrifuge Eppendorf
Centrifuge Eppendorf 5415 R
Lyophilizer
Thermomixers
Focused ultrasound probes
Microwave for digestion of samples

The group has access to all services of centers of support for research among which include the CAI of microscopy (very useful for characterization of NPs, and evaluation of cytotoxicity by flow cytometry).

UNED-QA

Liquid chromatograph with diode array (DAD), fluorescence and conductimetric detection.
Gas chromatograph coupled to mass spectrometry
Liquid chromatograph coupled to mass spectrometry.

Microwave

Spectrophotometer UV-VIS
Spectrofluorometer

Lab-284

ASAP 2020 Micromeritis equipment

Liquid chromatograph coupled to mass spectrometry Varian 1200/1200L

Capillary electrophoresis equipment P/ACETM MDQ from Beckman Coulter

UHPLC Dionex coupled to mass spectrometry ion trap from Bruker

Lab 147

In the Center are eight analytical laboratories with different equipments. Some of the equipments that are available are listed below:

Plasma Emission Spectrometer ICP, Liberty Axial Serie II, Spectrophotometer UV-Vis (Model Cary I-Bio), Spectrophotometer of fluorescence (Model Cary Eclipse).
Gas chromatograph GC (Model Star CX 3800/AS 8200) and head-space (Modelo Saturn 2000)
Gas chromatograph 7890B coupled to mass spectrometry 5977A from Agilent
6 Liquid chromatographs HPLC with DAD detection
Chromatograph HPLC with DAD and fluorescence detection
Chromatograph HPLC with ELS detection
Preparative Chromatograph HPLC Dynamax with 2 bombs SD-1
HPLC-MS Agilent 1100-MSD-20
Capillary Electrophoresis system Agilent CE
HPLC coupled to Triple Quad LC/MS de Agilent 6410
Ionic chromatograph 850 Metrohm
Spectrophotometer IR, Nicolet Impact-410
Viscometer Schott Visco Easy Plus.
Rheometer Haake Mars.
Differential Scanning Calorimeter Mettler-Toledo DSC-822
Microwave Milestone.
Titrator 702SM Tritino Metrohm
Titrator Karl-Fischer 701KF Tritino Metrohm
KF Coulometrico 831 Metrohm con Thermoprep 860
3 climate chambers Binder 700 L
Fash Biotage SP-1 system
Lyophilizer

Service Offer

The groups that are part of the program have their human resources dedicated to research and teaching at university level. This limits their ability to offer services for routine analysis to external sectors, as some analytical companies on the market make. On the other hand, the potential of knowledge and infrastructure that is accumulated in the program, should be used in their social environment, while retaining their main objectives of research and university teaching. All the media and the results obtained with the same will be screened on the following groups:

a) The scientific community, through counseling and the instrumental facilities to other groups that request them and, of course, by communicating of the obtained results in scientific journals and conferences.

b) The business sector, with which it is possible to establish agreements and contracts for delivery of analytical advice services when the company in question does not have the means to solve their problems. The wide variety of problems associated with the establishment of quality and safety in the food industry and the sheer volume of regulations and legislation to be met by food, seems to indicate that this collaboration can be very useful.

c) The Administration, in its various local, regional and national levels. The cooperation of the program with the various bodies responsible for ensuring the purity and quality of food can be very useful on a regular basis and especially when some kind of food scares appear as, unfortunately often happens with some frequency. The program can be a suitable tool and ready to establish and operate appropriate technical measures immediately, to solve the problems arising.